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New Slides (click on slide to enlarge)

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Alexa Dyes, New Fluorescent Dyes
    JHC 1999 47: 1179-1188.  [Abstract] [Full Text]
Apoptosis
    Cytochemical detection of Apoptosis JHC 1999 47: 1101-1110. [Abstract] [Full Text] 
    Advances in detection of Apoptosis JHC 2001 49: 821-832.[Abstract] [Full Text]  
    Apoptosis and cleaved Caspase 3 JHC 2002 50: 449-454.[Abstract] [Full Text]    
Antigen Retrieval
    AR: past, present and future JHC 1997 45: 327-344.  [Abstract] [Full Text]
    AR current perspectives JHC 2001 49: 931-938.   [Abstract]  [Full Text]
Immunofluorescence 
   Lipofuscin autofluorescence JHC 1999 47: 719-730.  [Abstract] [Full Text]   
   Autofluorescence in CLSM JHC 2001 49: 1565-1572. [Abstract] [Full Text]
   Intracellular signaling JHC 2002 50: 289-303.  
[Abstract] [Full Text]
    Reduction of autofluorescence JHC 2002 50: 437-439. [Abstract] [Full Text]    
Immunogold
    New frontiers in gold labeling JHC 2000 48: 471-480. [Abstract] [Full Text]    
   Blocking agents for immunogold labeling JHC 2002 50: 863-874.  [Abstract] [Full Text]  

In Situ Hybridization

    CARDS JHC 1999 47:281-288.[Abstract] [Full Text]        

    Co-labeling using In Situ PCR JHC 2001 49:1329-1340.[Abstract] [Full Text]  
   Whole-mount ISH JHC 2001 49: 1-8. [Abstract] [Full Text]    
    Improved non-radioactive ISH JHC 2002 50: 541-548. 
[Abstract] [Full Text] 
    Wilcox, Ph.D. 2000 [Full Text]
Specificity Controls
    JHC 2000 48: 163-166. [Abstract] [Full Text]
Stem Cell
   Stem cells in muscle repair JHC 2002 50: 589-610.  [Abstract] [Full Text]   
 
 
 
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In Situ Hybridization
    JHC 1993 41: 1725-1773.  [Full Text]
Tyramide Signal Amplification Enhanced Immuno-
histochemical and In Situ Hybridization Detection
    Roth, M.D., Ph.D. 2000 [Full Text]
Quantitative Immunohistochemistry by Measuring
Cumulative Signal Strength Using Commercially
Available Software Photoshop and Matlab
    JHC 2000 48: 303-312.   [Full Text]  
The Animal Research Kit (ARK) Can Be Used in a
Multistep Double Staining Method for Human Tissue
Specimens
    JHC 2000 48: 1431-1438.  [Full Text]
An Antigen Retrieval Method using an Alkaline 
Solution allows Immunoelectron Microscopy 
Identification of Secretory Granules in Epoxy
    JHC 2003 51: 199-204.  [Full Text]
A New Blocking Method for Application of Murine
Monoclonal Antibody to Mouse Tissue Sections
    JHC 1998 46: 977-984. [Full Text]    
 
 
 
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Discussion Board (coming soon)
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If you have useful techniques and procedures you want to 
share, please contact us at h.multhaupt@imperial.ac.uk
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Do you want to support the Histochemical Society by 
becoming a member?
You can join the Society by applying for the online membership or by sending the Membership Application form to:

The Histochemical Society, Dr. W.L. Stahl, Exec. Director; Dept Neurology, Box 356465; University of Washington; Seattle, WA 98195; Fax (206) 764 2164

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Expression of very low density lipoprotein receptor mRNA in arterioles. The mRNA are detected by fluorescent in situ hybridization (FISH), the nuclei are counterstained with propidium iodide.

Hinke A.B. Multhaupt Ph.D.

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Ultrastructural photomicrograph of a human lymphocyte.

Gary A. Stopyra, M.D.

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Primary fibroblast double stained with Texas Red phalloidin and FITC vinculin. Microfilament bundles terminating at focal adhesions.

John R. Couchman, Ph.D.

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Human kidney biopsy stained for IgG (FITC), shows diffuse thickening of the basement membrane of the glomerulus consistent with Lupus nephritis.

Hinke A.B. Multhaupt Ph.D.

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Confocal Laser Scanning photomicrograph of a pleural effusion of human epithelial cells stained with Epithelial Membrane Antigen (FITC). The nuclei are counterstained with Propidium Iodide.

Hinke A.B. Multhaupt Ph.D.

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Morphological features of apoptotic cells in culture detected by phase-contrast microscpoy. Characteristic features of apoptotic cells including blebs, echinoid spikes, and surface blisters.

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Successive staining of MCF7 cells with  FITC-Rodamine dextran followed by AO. (A) Photonic microscopy image. (B) Image of the intensity ratio after FRD.   (C) Image of R% after AO staining.

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GFAP immunoreactive astrocytes (orange) and neuronal nuclear antigen immunoreactive neurons (green), detected in the mouse hypothalamic periventricular region with multi-label Tyramide Signal Amplification (TSA) techniques

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Human LNCap cells treated with Adriamycin. Microtubules are identified with anti-alpha-tubulin antibody (anti-mouse labeled Alexa 488 secondary antibody); nuclei stained with TOTO-3.

 William Stahl, Ph.D.

 

 

 


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